Abstract: The scale unit is one of the most important factor in studying the measure of socio-spatial segregation. But few studies on the socio-spatial segregation in the past have a more comprehensive study about difference in scale of socio-spatial segregation. According to featuring the segregation profile and the corresponding macro/micro segregation ratio which offers a scale-sensitive alternative to standard methodological practice for describing segregation, the paper studies six occupational socio-spatial differentiation of Guangzhou. Using contour maps and measurement of five dimensions segregation (evenness, exposure, concentration, centralization, clustering) we gain patterns of occupational socio-spatial differentiation of Guangzhou. First, we find that evenness, concentration and clustering are greatly affected by scale change, but exposure and centralization are little a scale-sensitive. Spatial distribution of state organs, party and mass organizations, enterprises and institutions responsible person is the structure of two peaks that one is on the central urban area and other is on the southern suburbs of the city center. It has great evenness, concentration, centralization, but less exposure and clustering. Spatial distribution of handle affairs personnel and professional and technical personnel is the structure of single core and multicenter, and its evenness, concentration, centralization are great and exposure and clustering is little. Spatial distribution of business, service personnel is the structure of two peaks that one is on the central urban area and other is on the north suburbs of the city center and its five dimensions all is large. Spatial distribution of the production of transport equipment operators is like a volcano shape, which has a great of exposure, concentration, clustering and a little of evenness, centralization. Spatial distribution of farmers, forest guards, shepherd, fisherman, water conservancy production personnel like the shape of the basin, which is concentrated on the edge of the city area. It has great exposure and little evenness, concentration, centralization, clustering.