Abstract: In recent years, the micro-transformation mode, which is based on preserving the main status quo, has been proposed. The influence and effectiveness of the micro-transformation mode are constantly being disputed and discussed. Enning Road in Guangzhou is a historic district with profound cultural heritage in Xiguan, and its regeneration program has always been a research hotspot. It is believed that visual material can convey spatial practice experiences beyond language, by which people can explore the metaphor of space. Visual methodology combined with content analysis, semiology and measurement of emotion had been used to study the spatial perception of the micro-transformation space in Enning Road. The main goal is to explore the influence of the micro-transformation mode and attempt to reveal the spatial perception mechanism of historic district transformation. The study result shows that the perceived intensity of micro-transformation area is the highest. The satisfaction of basic needs offers good emotional experience. And the participatory festival activities can enhance the perception of the district. While the texture maintenance and cultural details of the buildings can preserve the sense of history and trigger the emotion of nostalgia. However, under the influences of the micro-transformation, like historic district’s material substitution and function transformation, the involvement of extraneous elements in spatial perception practice leads to changes in cultural code and different spatial perceptions. This study attempts to use the content analysis method to refine the visual perception traits, and quantify the differences of spatial perception before and after the transformation in way of emotion measurement. In addition, the study adopts new methods in the field of spatial perception.