Abstract: By using the spatial statistics data of China medicine manufacturing and combining with the relevant data of 31 provinces in the mainland China, we utilized the analysis method of GIS spatial statistics to establish the evaluation model of industrial layout, and then studied the spatial distribution characteristics and the influencing factors of industrial layout as well as the comprehensive layout of 31 provinces in mainland China. The results showed that: Firstly, the enterprise number in southeast China is much more than that in the northwest, which fits the Heihe-Tengchong as the dividing line. Secondly, the influence factors of industrial layout include three subsystems of society, economy and nature, and to optimize the industry layout could improve the development level of each sub-system and promote the balanced development of the three subsystems. Thirdly, the manufacture industry of China medicine in the eastern coastal areas as well as the extension areas to coastal inland in China develops well and its distribution is reasonable. However, both the comprehensive layout index and the coordination index of the manufacture industry in the western region of China were lower, which showed that the development level is worse and the distribution is unbalanced for the manufacturing industry.