Abstract: Based on the spatio-temporal data of game enterprises in Shanghai from 2006 to 2016, this article analyzes the spatial agglomeration and evolution of game industry in Shanghai using ArcGIS10.2 and Crimestat3.3, and reveals the main factors that influence its spatial agglomeration. The results show：(1) The spatial agglomeration feature of Shanghai game enterprises is obvious, and the evolution of "dual core agglomeration" to "single core agglomeration" is presented. (2) There is a double trend of agglomeration and diffusion of game enterprises. As the enterprises spread to the periphery of Jiading and other cities, agglomeration of Pudong and Xuhui is further strengthened. (3) The intensity of agglomeration between the main agglomeration areas is positively correlated with the size of agglomeration. (4) The factors that affect the spatial agglomeration of game enterprises in Shanghai are multifaceted. The spatial location demand of enterprises is the basic factor. The spatial location requirements of enterprises are the basic factors for the location of enterprises. Government policies and planning are important guiding factors in attracting and changing the spatial pattern of enterprises. The advantages of talents and capital in industrial bases and parks have become another important reason for the agglomeration of game enterprises.