Abstract: Geo-culture is one of incentives of the Ukraine Crisis, which influences domestic and foreign affairs in Ukraine. Ukraine plays a significant strategic role for Chinese “One Belt and One Road” initiative as a geopolitical fulcrum country. Research on geo-culture characteristics and influence is crucial to distinguish geopolitics and geo-economy characteristics in Ukraine. This paper aims to explore the spatial statistic pattern of geo-culture characteristics in Ukraine (except cities of Kyiv and Sevastopol) and analyze its influence on foreign trade relations and political trends applying spatial autocorrelation based on Ukraine population data, foreign trade volume data and presidential candidate’s vote share data. Conclusions are as follows: (1) Geo-culture in Ukraine exist obvious spatial aggregation characteristics to cut apart geographic spatial of Ukraine. (2) Eastern region has the stronger foreign trade relations with many Russian; Western region has the weaker foreign trade relations with bare Russian. (3) Western region is pro-Western presidential candidate’s sphere of influence with numerous Ukrainian; Eastern region is pro-Russia presidential candidate’s sphere of influence with many Russian.